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【文獻標題】Transcriptome Characterization of Cymbidium sinense'Dharma' Using 454 Pyrosequencing and Its Application in the Identification of Genes Associated with Leaf Color Variation

【作者】Genfa Zhu，F(xiàn)engxi Yang，Shanshan Shi，et.al

【作者單位】華中農(nóng)業(yè)大學（Huazhong Agricultural University）

【文獻中引用產(chǎn)品】

植物葉綠素（chlorophyll）ELISA試劑盒

【DOI】DOI:10.1371/journal.pone.0128592

【影響因子(IF)】3.02

【出版期刊】《PLOS ONE》

【產(chǎn)品原文引用】

Biochemical measurements

For Chlorophyll content measurement, six-month old leaves of wild type and the mutant grown under identical conditions were collected and Chl (a,b) were extracted in 90% acetone and determined spectrophotometrically by the method of Lorenzen [26]. For the contents of chlorophyll intermediate products, leaves were homogenized in nine volume of 0.01 M Phosphate Buffered Saline (PBS), and mixed on the ice and centrifuged (30 min at 2,500g). The supernatants was assayed by a ELISA kit (HengYuan Biological Technology Co.,Ltd, Shanghai,China) separately. Data were analyzed by SPSS software.

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